Argonne National Laboratory
The Advanced Photon Source (APS) at the U.S. Department of Energy’s Argonne National Laboratory provides ultra-bright, high-energy storage ring-generated x-ray beams for research in almost all scientific disciplines. The APS is conveniently located at Argonne National Laboratory, 30 miles southwest of Chicago, Illinois, with two nearby airports offering many travel options.The unique properties of the APS synchrotron radiation are its continuous spectrum, high flux and brightness, and high coherence, which make it an indispensable tool in the exploration of matter. The wavelengths of the emitted photons span a range of dimensions from the atomic level to biological cells, thereby providing incisive probes for advanced research in materials science, physical and chemical sciences, metrology, geosciences, environmental sciences, biosciences, medical sciences, and pharmaceutical sciences.There are three broad categories of synchrotron experimental measurement techniques available at the APS that are listed below:
The x-ray beam is customized at each beamline to meet particular needs. With more than 60 beamlines operational, the APS offers an exceptionally broad range of experimental conditions at a single facility.
Each beamline at the APS offers a unique combination of capabilities, but some of the main considerations are energy range and tunability, special sample environments, time structures, and beam size. Specifics for each beamline and a directory of techniques can be found in the Beamlines section; however, the techniques and capabilities evolve continually. The following are some highlights.
There are a number beamlines and x-ray techniques available at the APS. The complete listing of all APS beamlines’ contacts, specifications, and status is available at the APS website. Access to these beamlines is available through the general user program.
The DOE-BER funded Structural Biology Center (SBC) is a scientific user facility for macromolecular crystallography and at Sector 19 of the Advanced Photon Source (APS). The SBC’s two beamlines – one insertion device (19-ID) and one bending magnet (19-BM) – provide highly powerful, capable and productive x-ray sources for structural biology in the US. They can deliver very low angular divergence x-ray micro-beams onto micrometer-size crystal samples mounted using a robotic systems, thereby permitting structural biologists to study the structures of large and complex molecular systems at atomic resolution. Diffraction from these crystals is recorded on large, fast, and efficient area detectors, and is processed on integrated computing systems with advanced control and data analysis software designed specifically for the SBC. The integrated robotics of sample mounting and the automation of data collection and structure determination lowers the training barrier for beamline use and allows remote access on both beamlines.
The 19-ID and 19-BM beamlines are highly efficient facilities for macromolecular crystallography covering a wide range of crystallographic projects. The SBC data demonstrate the great potential of third-generation light sources for high-throughput crystallography: (1) use of the small, brilliant undulator beam and mini-beams is highly efficient, (2) our new detector on 19-ID allows for shutter-less data acquisition for competing a data set in minutes and processing of data measured to the limit of crystal diffraction is done in near real time, (3) all aspects of the experiment, including crystal decay and the signal-to-noise ratio, can be optimized, (4) in many cases, data can be processed and structures determined using semi-automated approaches in near-real time and (5) the in situ and in cellulo data collection is available on 19-ID.
The SBC beamlines offer one of the most efficient worldwide data collection and structure determination systems currently available for protein crystallography and have continued to demonstrate record productivity – 4902 PDB deposits and 1774 unique publications.
The SBC user program is fully synchronized with the APS proposal system, and peer review process. Users requesting beamtime must complete the proposal request form and submit it electronically through the APS website. All proposals are peer-reviewed by the APS review system. Beamtime is allocated based on the project’s priority score.
The SBC accepts the following categories of proposals:
SBC users can access the beam lines both on site and/or remotely for their experiments, structure analysis, and data backup.
The Advanced Protein Characterization Facility (APCF) is a community resource available to users. This new structural biology resource is located in the Bldg. 446 (a 65,000 sq. ft. building), directly attached to Sector 19 of the APS, where SBC beamlines are located. These laboratories include:
These resources are available on cost-recovery basis and they are shared with the Midwest Center for Structural Genomics resource and Center for Structural Genomics of Infectious Diseases. The access to the APCF services is available on the cost recovery basis. The APCF uses automation and robotics for gene cloning, protein expression, protein purification, characterization and protein crystallization. The complete HTP platform and an expert team working together for over a decade was established. It delivers high quality expression clones, purified proteins and structures at low cost. The APCF hosts eight highly integrated core-technology units:
Office space and conference rooms are available for users in Bldg 446. A conference room, several meeting rooms with teleconferencing capabilities are also available. The APCF hosts several computer clusters and has dedicated local servers for computing, databases, web application, and over 200 TB storage. Experimental data is stored in an Enterprise Oracle Database.
Structural Biology Center; Director
Midwest Center for Structural Genomics; PI
Center for Structural Genomics of Infectious Diseases
Argonne Distinguished Fellow
University of Chicago; Professor
Structural Biology Center
Biosciences Division, Bldg. #446; Room A-104,
Argonne National Laboratory,
9700 South Cass Avenue,
Argonne, IL 60439 USA
Phone: (630) 252-3926
Fax: (630) 252-0564