BER Structural Biology and Imaging Resources
Synchrotron, Neutron, and Cryo-EM
U.S. Department of Energy | Office of Science | Office of Biological and Environmental Research

Cellulose Synthesis Complex

January 12, 2016

Feature Story

The plant cellulose synthesis complex is a large multi-subunit transmembrane protein complex responsible for synthesis of cellulose chains and their assembly into microfibrils. The image shows ab initio structures of CESA trimers calculated from small-angle scattering data represented by semi-transparent grey surface envelopes, superposed with the computational atomic models in orange. Image credits: Thomas Splettstoesser,, Berlin Germany

Vandavasi, V.G., D.K. Putnam, Q. Zhang, L. Petridis, W.T. Heller, B.T. Nixon, C.H. Haigler, U. Kalluri, L. Coates, and P. Langan. 2016. “A structural study of CESA1 catalytic domain of Arabidopsis cellulose synthesis complex: evidence for CESA trimers.” Plant physiology 170(1):123-35. [DOI: 10.1104/pp.15.01356]

Funding Acknowledgements: Molecular biology and structural characterization: H.O., V.G.V., Q.Z., W.T.H., L.P., U.K., J.C.S., P.L., and L.C., supported by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory (ORNL), managed by UT-Battelle, LLC, for the U.S. Department of Energy (DOE) under Contract Number DE-AC05-00OR22725. Small-angle scattering (SAS) and computational analysis, performed by H.O., V.G.V., L.P., B.T.N., and C.H.H., supported by Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center (EFRC) funded by the Office of Basic Energy Sciences (OBES). DOE Office of Science. J.M. and D.K.P. support: High Performance Computing Grant from Oak Ridge Associated Universities (ORAU). Bio-SANS is operated by the Center for Structural Molecular Biology at ORNL, supported by Office of Biological and Environmental Research (OBER), DOE Office of Science, Project ERKP291. EQ-SANS at Spallation Neutron Source (SNS) and High Flux Isotope Reactor (HFIR) sponsored by OBES Scientific User Facilities Division, DOE Office of Science, at ORNL. Sai Venkatesh Pingali: assistance with the operation of Bio-SANS and data reduction. Paul Abraham and the BioEnergy Science Center (BESC) proteomics facilities: validation of purified proteins. BESC supported by DOE OBER. Mass spectrometry (MS) analysis carried out by DOE OBER?supported Bioenergy Research Center proteomics pipeline. Use of the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory (BNL), supported by OBES, DOE Office of Science, under Contract Number DE-AC02-98CH10886.