Essential Enzyme in Bacterial Protein Synthesis
In recent work using the Structural Biology Center X-ray beamline 19-ID at the U.S. DOE’s Advanced Photon Source, researchers gained important insights into the structure and mechanism of phenylalanyl-tRNA synthetase, a critical enzyme involved in protein synthesis in Mycobacterium tuberculosis.
Multi-drug resistant and extensively drug-resistant strains of M. tuberculosis have become a major global problem, increasing the need for new antibiotics. This has driven researchers to seek new bacterial targets for structure-based drug design.
The work, published in Nucleic Acids Research, will form the basis of structure-based drug design efforts aimed at exploiting the unique features of the enzyme, a method that has been successful in developing pharmaceuticals against methicillin-resistant Staphylococcus aureus (MRSA) and some fungal infections.
- BER Resource: Structural Biology Center
- Feature Story: Using Protein Structural Information to Understand the Mechanism of an Essential Enzyme for Fighting Tuberculosis
Michalska K, Jedrzejczak R, Wower J, Chang C, Baragaña B, Gilbert IH, Forte B, Joachimiak A. “Mycobacterium tuberculosis Phe-tRNA synthetase: structural insights into tRNA recognition and aminoacylation,” Nucleic Acids Res. 49(9):5351-5368. [DOI: 10.1093/nar/gkab272]