First X-ray Nanofluorescence Tomography of Single Bacterial Cell
The elemental distribution and nanostructure of single E. coli cell were co-localized simultaneously using X-ray fluorescence (XRF) nanotomography and ptychography using a sub-15 nm beam. This multimodal approach presents new possibilities in understanding subcellular biochemistry in individual organelles, which are usually analyzed at the population level.
First X-ray Nanofluorescence Tomography of Single Bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria’s chemical composition.
T. W. Victor, L. M. Easthon, M. Ge, K. H. O’Toole, R. J. Smith, X. Huang, H. Yan, K. N. Allen, Y. S. Chu, L. M. Miller. “X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam.” Scientific Reports. 8: 13415 (2018). [DOI: 10.1038/s41598-018-31461-y]