Goodell, B., et al. “Modification of the Nanostructure of Lignocellulose Cell Walls via a Non-Enzymatic Lignocellulose Deconstruction System in Brown Rot Wood-Decay Fungi.” Biotechnol. Biofuels 10(1), 179 (2017). [DOI:10.1186/s13068-017-0865-2].
Instruments and Facilities Used: Small angle neutron scattering (SANS) – Bio-SANS at High Flux Isotope Reactor (HFIR) at Oak Ridge National Laboratory (ORNL). Sum frequency generation (SFG) spectroscopy, broadband SFG system. Chelator-mediated Fenton treatments (CMF) and cellulase treatment. X-ray diffraction analysis (XRD) – PANalytical Empyrean diffractometer, The Netherlands, equipped with a Cu X-ray source. Attenuated total reflectance Fourier transform infrared analysis (ATR-FTIR): Nicolet 8700 FTIR Spectrometer (Thermo Scientific) equipped with a smart iTR diamond ATR unit, a KBr beam splitter, and a deuterated triglycine sulfate (DTGS) detector. Atomic force microscopy (AFM) of brown-rotted wood surfaces. Nanoscope IIIa AFM-Digital Instruments, Santa Barbara, California with three 5 µm scans. Transmission electron microscopy (TEM) – Philips CM12 TEM instrument (Philips, Eindhoven, The Netherlands); images recorded on Kodak 4489 negative film and the films subsequently scanned using an Epson Perfection Pro 750 film scanner.
Funding Acknowledgements: Dr. Zhu support: Chinese Forestry Industry Research Special Funds for Public Welfare Projects (#201204702-B2). Eastwood support: UK Natural Environment Research Council, award #NE/K011588/1. Daniel support: Formas Grant 2015-469. Support from ORNL-Proposal IPTS-12345/CG-3. Sum frequency generation (SFG) spectroscopy, x-ray diffraction (XRD), and infrared (IR) studies support: Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center, funded by the Office of Basic Energy Sciences (OBES), U.S. Department of Energy (DOE) Office of Science, under Award Number DE-SC0001090. Pingali and O’Neill support: Biofuels SFA funded by the DOE Genomic Science Program, Office of Biological and Environmental Research (OBER), under Contract FWP ERKP752. Bio-SANS support: Center for Structural Molecular Biology, supported by DOE OBER under Contract FWP ERKP291. Neutron scattering facilities at Oak Ridge National Laboratory (ORNL) support: Scientific User Facilities Division, DOE OBES. Research also supported by U.S. Department of Agriculture’s (USDA) National Institute of Food and Agriculture (NIFA) and University of Massachusetts Amherst’s Center for Agriculture, Food and the Environment and the Microbiology Department: project # MAS00511.