New Approach for Solving Protein Structures from Tiny Crystals
March 3, 2019
Using X-rays to reveal the atomic-scale three-dimensional structures of proteins has led to countless advances in understanding how these molecules work in bacteria, viruses, plants, and humans. To grow some proteins into crystals large enough for this approach, scientists at the U.S. Department of Energy’s (DOE) Brookhaven National Laboratory (BNL) and colleagues at Columbia University have developed a new approach for solving protein structures from tiny crystals. To examine previously inaccessible microcrystals, they are using unique sample-handling, signal-extraction, and data-assembly approaches, along with a beamline capable of focusing intense X-rays at BNL’s National Synchrotron Light Source II.
Guo, G., et al. “Sample Manipulation and Data Assembly for Robust Microcrystal Synchrotron Crystallography.” IUCrJ 5(Part 3) 238–46 (2018). [DOI: https://doi.org/10.1107/S2052252518005389]