Symbiotic associations in the rhizosphere influence the distribution of nutrients that promote the growth and development of both plants and microbes. Fourier-transform infrared imaging (FTIR) shows evidence of symbiotic exchange of nitrate and sucrose between a poplar root and fungi in the poplar rhizosphere (A). Nitrate concentration (B) is elevated in regions of high fungal colonization and decreases away from the plant root as the fungal population decreases. Sucrose concentration, on the other hand, (C) increases. The trend suggests that there is symbiotic sharing of nutrients between the plant and the fungi, where the fungi consume the sucrose and sequester nitrate for the plant’s use. [Reprinted with permission from Victor, T., et al. 2017. "Imaging Nutrient Distribution in the Rhizosphere Using FTIR Imaging," Analytical Chemistry 89(9) 4831–37. DOI:10.1021/acs.analchem.6b04376. Copyright 2017 American Chemical Society.]
Fourier-transform infrared imaging (FTIR), or FTIR spectromicroscopy, correlates infrared maps with visible microscopy images to map the distributions of molecular compositions of interest within biological samples and to reveal sample morphology and structure. Compared to desktop FTIR chemical imaging techniques, synchrotron-based FTIR (sFTIR) can provide detailed images of the location and concentration of molecules with a resolution of ~2.0 to ~10 µm per pixel and a sensitivity 100- to 1,000-X. The BER-supported sFTIR resource at Lawrence Berkeley National Laboratory’s Advanced Light Source also has multiple time-resolved microfluidic sFTIR imaging capabilities for tracking the location and dynamics of biochemical processes in live cells or biofilms. This can be applied to study chemical events in real time on a timescale of seconds and longer. Sample requirements and preparation are generally minimal, enabling diverse user experiments and the study of wide-ranging science questions.
Bacterial components in an algal system might play relevant roles in algal plasticity and adaptive responses to a changing environment. Synchrotron-based Fourier-transform infrared spectromicroscopy (sFTIR) reveals the distributions of dissolution-resistant, high-magnesium carbonate minerals and other compounds involved in biomineralization inside a single cell of a marine coralline algae. This suggests diverse metabolic function by symbiotic microbes which may help confer resilience to ocean acidification. [Reprinted under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International. https://creativecommons.org/licenses/by-nc-nd/4.0/ from Valdespino-Castillo, P. M., et al. 2021. "Interplay of Microbial Communities with Mineral Environments in Coralline Algae," Science of The Total Environment 757, 143877. DOI: 10.1016/j.scitotenv.2020.143877.]
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